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Reactive Oxygen Species of Ozonolysis Products of Some Unsaturated Organic Compounds

Reactive Oxygen Species of Ozonolysis Products of Some Unsaturated Organic Compounds

Gordetsov А.S., Peretyagin S.P., Kadomtseva А.V., Novikova А.N., Grechkaneva О.V., Zimina S.V.
Key words: Fourier-transform IR spectroscopy; ozone; hydroperoxyacids; hydroperoxyesters.
2019, volume 11, issue 3, page 60.

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The aim of the investigation was to study the composition of reaction systems formed when certain lipids are ozonized, in particular, olive oil lipids and olive oil model system — oleic acid, as well as to reveal reactive oxygen species (ROS) in the systems resulting in sanogenetic properties appearing in preparations based on the properties.

Materials and Methods. Lipids containing oleic acid in esterified and free forms were ozonized by an ozone therapy unit with an ozone destructor (Medozons-03, AOT-N-01-Arz-01, Russia). The chemical composition and structure of ozonolysis products containing ROS were studied using Fourier-transform IR spectrophotometer (Shimadzu IR Prestige 21, Japan) in a wave number area 4000–400 cm–1 in the form of liquid films in the windows from KBr or ZnSe. ROS were searched by comparing IR spectra of studied samples before and after ozonolysis. The dynamics of ROS formation and concentration changes were also controlled by iodometric titration according to Interstate standard GOST ISO 3960-2013. The properties of medicinal preparations made on ozonized products, as well as ROS concentration changes were studied in a similar way.

Results. Fourier-transform IR spectroscopy enabled to find that the ozonolysis of lipids containing oleic acid in esterified and free forms results in the formation of hydroperoxyacids and hydroperoxyesters, respectively, rather than ozonides, as basic relatively stable ROS forms. The concentration of ozonides is rather low, while hydroperoxy-derivatives segregate rather slowly, and exist in reaction products (systems) within several months. Multi-component hydrophilic bases of pharmaceuticals have a slight direct chemical effect on this ROS type.

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Gordetsov А.S., Peretyagin S.P., Kadomtseva А.V., Novikova А.N., Grechkaneva О.V., Zimina S.V. Reactive Oxygen Species of Ozonolysis Products of Some Unsaturated Organic Compounds. Sovremennye tehnologii v medicine 2019; 11(3): 60, https://doi.org/10.17691/stm2019.11.3.08


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