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Application of Dimethicone to Prevent Culture Media from Drying in Microbiological Diagnostics

Application of Dimethicone to Prevent Culture Media from Drying in Microbiological Diagnostics

Savinova T.A., Bocharova Y.A., Mayansky N.A., Chebotar I.V.
Key words: bacteria growth and generation; bacterial mobility; culture media; microorganism cultivation; dimethicone.
2023, volume 15, issue 1, page 14.

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The search for novel modifications of culture media aimed at culture prolongation is a prerequisite for microbiological diagnostic progress.

The aim of the study was to assess the possibilities of applying dimethicone (polymethylsiloxane) as a barrier between the agar surface and atmosphere to prevent drying of solid and semisolid culture medium providing the retention of its useful properties.

Materials and Methods. We studied the dynamics of water (volume) loss of culture media used in microbiology, and the effect of dimethicone on the process. Dimethicone was arranged in layers on culture medium surface. The effect of dimethicone on growth and generation of fast-growing (Staphylococcus aureus, Escherichia coli, Salmonella enterica Serovar Typhimurium, Burkholderia cenocepacia) and slow-growing (Mycobacterium avium) bacteria was studied, as well as on bacterial mobility (Pseudomonas aeruginosa and Escherichia coli) in semisolid agars.

Results. The dynamics of water loss in culture media showed the weight loss in all media without dimethicone (control) in 24 h to be statistically significant (p<0.05); 7–8 days later, they lost 50% of weight, and 14 days later they lost approximately 70%. The weight of media under dimethicone underwent no significant changes during the observation period. Growth index of fast-growing bacteria (S. aureus, E. coli, S. Typhimurium, B. cenocepacia) on control culture media without applying any substance, and on culture media under dimethicone had no significant differences. Visible M. avium growth on chocolate agar in controls was recorded on day 19, under dimethicone — on days 18–19. The number of colonies on culture day 19 under dimethicone tenfold exceeded the control values. The mobility indices of P. aeruginosa and E. coli on semisolid agar under dimethicone 24 h later were significantly higher than under control conditions (p<0.05 in both cases).

Conclusion. The study confirmed marked deterioration of culture media properties under prolonged cultivation. The suggested protection technology of culture media growth properties using dimethicone showed beneficial effects.

  1. Stahl D.A., Urbance J.W. The division between fast-and slow-growing species corresponds to natural relationships among the mycobacteria. J Bacteriol 1990; 172(1): 116–124, https://doi.org/10.1128/jb.172.1.116-124.1990.
  2. Morris C., Lee Y.S., Yoon S. Adventitious agent detection methods in bio-pharmaceutical applications with a focus on viruses, bacteria, and mycoplasma. Curr Opin Biotechnol 2021; 71: 105–114, https://doi.org/10.1016/j.copbio.2021.06.027.
  3. Chebotar’ I.V., Emelyanova M.A., Bocharova J.A., Mayansky N.A., Kopantseva E.E., Mikhailovich V.M. The classification of bacterial survival strategies in the presence of antimicrobials. Microb Pathog 2021; 155: 104901, https://doi.org/10.1016/j.micpath.2021.104901.
  4. Sandle T. Settle plate exposure under unidirectional airflow and the effect of weight loss upon microbial growth. Eur J Parenter Pharm Sci 2015; 20(2): 45–50.
  5. Lyamin A.V., Тoneev I.R., Kozlov A.V., Khismatullin D.D., Zhestkov A.V., Kondratenko O.V., Kovalev A.M. Bottle for the cultivation of micro-organisms. Patent RU 175134. 2017.
  6. XIAMETER™ PMX-200 Silicone Fluid 350 cSt. Technical Data Sheet. The Dow Chemical Company; 2017. URL: https://www.dow.com/en-us/pdp.xiameter-pmx-200-silicone- fluid-350-cst.01013211z.html?productCatalogFlag=1#tech-content.
  7. Cannon P., St. Pierre L.E., Miller A.A. Solubilities of hydrogen and oxygen in polydimethylsiloxanes. J Chem Eng Data 1960; 5(2): 236, https://doi.org/10.1021/je60006a027.
  8. Clark L.C. Jr., Gollan F. Survival of mammals breathing organic liquids equilibrated with oxygen at atmospheric pressure. Science 1966; 152(3730): 1755–1756, https://doi.org/10.1126/science.152.3730.1755.
  9. Nair B.; Cosmetic Ingredients Review Expert Panel. Final report on the safety assessment of stearoxy dimethicone, dimethicone, methicone, amino bispropyl dimethicone, aminopropyl dimethicone, amodimethicone, amodimethicone hydroxystearate, behenoxy dimethicone, C24-28 alkyl methicone, C30-45 alkyl methicone, C30-45 alkyl dimethicone, cetearyl methicone, cetyl dimethicone, dimethoxysilyl ethylenediaminopropyl dimethicone, hexyl methicone, hydroxypropyldimethicone, stearamidopropyl dimethicone, stearyl dimethicone, stearyl methicone, and vinyldimethicone. Int J Toxicol 2003; 22(Suppl 2): 11–35.
  10. Moore K., Bolduc S. Prospective study of polydimethylsiloxane vs dextranomer/hyaluronic acid injection for treatment of vesicoureteral reflux. J Urol 2014; 192(6): 1794–1800, https://doi.org/10.1016/j.juro.2014.05.116.
  11. Lam R.H.W., Kim M.C., Thorsen T. Culturing aerobic and anaerobic bacteria and mammalian cells with a microfluidic differential oxygenator. Anal Chem 2009; 81(14): 5918–5924, https://doi.org/10.1021/ac9006864.
Savinova T.A., Bocharova Y.A., Mayansky N.A., Chebotar I.V. Application of Dimethicone to Prevent Culture Media from Drying in Microbiological Diagnostics. Sovremennye tehnologii v medicine 2023; 15(1): 14, https://doi.org/10.17691/stm2023.15.1.02


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