Atrial and Brain Natriuretic Peptides of Secretory Cardiomyocytes in Salt Loading in Experiment

The aim of the investigation is to assess the influence of salt load on atrial (ANP) and brain (BNP) natriuretic peptide production in granules of secretory cardiomyocytes in rats.

Materials and Methods. The experiments were carried out on 14 white out-bred male Wistar rats weighing 280–300 g. During the experiment all the animals were treated with standard-feed diet and had unlimited access to food and water. NaCl solution was introduced per os in the dose of 1 g per 1 kg of body mass during 14 days. Arterial pressure (AP) was measured noninvasively using a tail-cuff method. ANP and BNP production of atrial cardiomyocytes was studied by means of immunohistochemistry, transmission electron microscopy, immunocytochemistry. There was performed a morphometric analysis of granules containing peptides (A-type — “mature, storing” and B-type — “dissolving”).

Results. Increase in the number of granules with ANP and decrease in those with BNP accompanied by elevated AP was revealed 14 days after NaCl intake as compared to intact animals.

Conclusion. Natriuretic peptides metabolism is regulated by various mechanisms. Early BNP release does not promote AP reduction due to compensatory mechanism disturbance in salt-induced arterial hypertension. Increase in ANP production occurs under the influence of renin-angiotensin-aldosterone system and elevated AP. The present data can indicate adaptive reaction in response to salt loading.

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Galkina М.V., Snopova L.B., Prodanets N.N., Lapshin R.D., Belousova I.I., Abrosimov D.A., Bugrova М.L. Atrial and Brain Natriuretic Peptides of Secretory Cardiomyocytes in Salt Loading in Experiment. Sovremennye tehnologii v medicine 2016; 8(3): 49, https://doi.org/10.17691/stm2016.8.3.05