Today: Aug 13, 2025
RU / EN
Last update: Jun 30, 2025
Transparent PEG-Fibrin Gel as a Flexible Tool for Cell Encapsulation

Transparent PEG-Fibrin Gel as a Flexible Tool for Cell Encapsulation

Shpichka A.I., Revkova V.A., Aksenova N.A., Yusubalieva G.M., Kalsin V.A., Semenova E.F., Zhang Y., Baklaushev V.P., Timashev P.S.
Key words: fibrin gel; cell encapsulation; PEGylation; matrix; fibroblasts.
2018, volume 10, issue 1, page 64.

Full text

html pdf
2400
2887

The aim of this study was to modify the chemical structure and to optimize the composition of the fibrin gel for effective cell encapsulation.

Materials and Methods. We prepared PEGylated fibrin gels using different fibrinogen concentrations (25–50 mg/ml) and PEG-fibrinogen molar ratio 10:1 and 5:1 and characterized them via Fourier transform infrared spectroscopy and differential scanning calorimetry. Within the gels, we encapsulated primary culture of fibroblasts and analyzed using light and laser confocal microscopy.

Results. PEGylation of fibrinogen allowed us to achieve the gel transparency and preserve its biocompatibility. We revealed that the gel prepared from PEGylated 5:1 fibrinogen (25 mg/ml) provided the most favorable microenvironment for spreading, growth, and proliferation of fibroblasts. This PEG-fibrin gel can be used for encapsulation of different cell types that is essential for various approaches in tissue engineering and diagnostic systems.


Journal in Databases

pubmed_logo.jpg

web_of_science.jpg

scopus.jpg

crossref.jpg

ebsco.jpg

embase.jpg

ulrich.jpg

cyberleninka.jpg

e-library.jpg

lan.jpg

ajd.jpg

SCImago Journal & Country Rank