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Biochip-SER Test System for Fluorescent Immunocytochemical Analysis of Ep-CAM Antigen Expression in Effusions and Washes

Biochip-SER Test System for Fluorescent Immunocytochemical Analysis of Ep-CAM Antigen Expression in Effusions and Washes

Zinoviev S.V., Terentiev I.G., Utkin O.V., Furminskaya E.Yu., Fedoseeva E.S., Savostikova M.V.
Key words: metastatic effusion; Biochip-SER test system; immunocytochemical study; fluorescent immunocytochemical study; Ep-CAM antigen; Ber-EP4; Alexa Flour 488.
2018, volume 10, issue 4, page 34.

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The aim of the study was to evaluate the Biochip-SER test system for its ability to measure the expression of Ep-CAM antigen by fluorescent immunocytochemistry.

Materials and Methods. We conducted 64 cytological, 64 standard immunocytochemical (SICC) and 64 fluorescent immunocytochemical (FICC) tests using the Biochip-SER test system and samples from 59 patients (45 effusions and 19 peritoneal washings). The study was performed in 4 stages: cytological examination, SICC, FICC using the Biochip-SER system, and control cytological study by the Biochip-SER system. The SICC was performed using the monoclonal antibody (MAB) to the epithelial antigen Ep-CAM; FICC was performed using the MAB to the epithelial antigen Ep-CAM conjugated to the Alexa Flour 488 fluorochrome.

Results. FICC test performed using the Biochip-SER test system, showed 100% diagnostic sensitivity and 89% specificity.

Conclusion. FICC test carried out with MAB to the Ep-CAM antigen conjugated with Alexa Flour 488 using the Biochip-SER system is a reliable method for diagnosing tumors by testing effusion fluids, which allows us to recommend this system for practical use in specialized institutions and primary points of care.


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